Polymerization of actin modified with fluorescein isothiocyanate
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منابع مشابه
Polymerization of ADP-actin
Using hexokinase, glucose, and ATP to vary reversibly the concentrations of ADP and ATP in solution and bound to Acanthamoeba actin, I measured the relative critical concentrations and elongation rate constants for ATP-actin and ADP-actin in 50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 0.1 mM nucleotide, 0.1 mM CaCl2, 10 mM imidazole, pH 7. By both steady-state and elongation rate methods, the critical co...
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763 The Rockefeller University Press $30.00 J. Cell Biol. Vol. 212 No. 7 763–766 www.jcb.org/cgi/doi/10.1083/jcb.201512019 Introduction Mechanical amplification is something we experience every day, in the form of gears, pulleys, and levers. While climbing a hill on a bicycle, for instance, shifting gears increases the force on the wheels while limiting the pressure required on the pedals. Howe...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1988
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1988.tb14057.x